Figure 3 .

Low levels of H4K16Ac are present at the centromere and at noncentromeric loci enriched for Cse4p. ChIP experiments were performed with asynchronously grown wild-type strains (YMB6955 in B and YMB8077 in C). Immunoprecipitated DNA was subjected to quantitative PCR to determine enrichment for the indicated regions. (A) Diagram of CEN III (C1–4) and pericentromeric regions (L1 and R1) analyzed by ChIP. (B) ChIP experiments with antibodies to acetylated H4K16 (H4K16Ac) and pan H4 to measure levels of H4K16Ac and total H4 at C1–4 and at L1 and R1 or in C at NTS1-1 and NTS1-2 rDNA regions that contain Cse4p. HML (−CTL1) and SPS22 (−CTL2) (Chr III: 42437–42630) serve as hypoacetylated and ARE1 (+CTL) (Chr III: 212230–212450) serves as hyperacetylated H4K16 controls. Bar graphs show mean fold enrichment for H4K16Ac normalized to total H4 from three biological replicates with error bars showing standard error of the mean.