Figure 4 .

NAM causes an increase in centromeric H4K16Ac in wild-type and cse4-1 strains. ChIP experiments were performed with CSE4 (RC154) and cse4-1 strains (RC147) grown in 0 mM or 30 mM NAM. Immunoprecipitated DNA was subjected to quantitative PCR to determine enrichment for H4K16Ac. (A) Diagram of CEN III and pericentromeric regions. (B) ChIP experiments with antibodies to H4K16Ac and pan H4 to measure levels of H4K16Ac at C1 in CSE4 strains. (C) Same as B except in cse4-1 strains. HML serves as a hypoacetylated H4K16 control region. Bar graphs show mean fold enrichment for H4K16Ac normalized to total H4 from three biological replicates with error bars showing standard error of the mean.