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. 2011 Sep;189(1):11–21. doi: 10.1534/genetics.111.130781

Figure 5 .

Figure 5 

Overexpression of SAS subunits leads to SDL in kinetochore mutants. Strains were transformed with vector (vector) or vector containing the indicated gene under the Gal1 promoter (GAL-). Fivefold serial dilutions of each strain were spotted on SC −Ura plates containing 2% galactose and 2% raffinose, incubated at the indicated temperatures, and photographed after 3–5 days. (A) Wild-type CSE4 (RC154) and C-terminal mutant cse4-1 (RC147). (B) C-terminal mutant cse4-111 (MSY1520). (C) N-terminal mutant cse4-39 (YC190). (D) Wild-type CSE4 (RC154) and C-terminal mutant cse4-1 (RC147). (E) Wild-type HHF1 (MSY559) and temperature-sensitive chromosome segregation mutant hhf1-20 (MSY554) strains. Three or more independent transformants were tested for each experiment.