Figure 6 .

Catalytic activity of Sas2p contributes to SDL and GAL-SCM3 suppresses the GAL-SAS2 SDL. Strains were transformed with vector (vector) or vector containing the indicated gene under the Gal1 promoter (GAL-). Fivefold serial dilutions of each strain were spotted on SC −Ura plates containing 2% galactose and 2% raffinose, incubated at the indicated temperatures, and photographed after 3–5 days. (A) C-terminal mutants cse4-1 (RC147) and cse4-111 (MSY1520) strains, carrying vector, GAL-SAS2, or the catalytic mutant GAL-sas2m1. (B) C-terminal mutant cse4-1, cse4-111, and histone H4 mutant hhf1-20 (MSY554) strains, carrying vector and GAL-SCM3 or GAL-SAS2 or carrying both GAL-SAS2 and GAL-SCM3. At least three independent transformants were tested for each experiment.