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Design of genetic screens. Wild-type hermaphrodites were mutagenized with EMS, F1 self-progeny were cultured individually, and the indicated number of F2 self-progeny was analyzed. F2 hermaphrodites were self-fertilized (A) or mated to wild-type males on the indicated days (B and C). F2 animals were analyzed for aging spans (A) or cumulative progeny production from day 10 until cessation (B and C). N indicates the number of F1 animals analyzed.
