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Relationship to the cAMP pathway. (A) CR-1 (adenylyl cyclase) protein levels. Samples containing 100 μg of protein from whole-cell extracts from 16-hr submerged cultures were subjected to Western analysis using the CR-1 antibody. Strains used were the same as in Figure 3A. (B) Effects of the mcb mutation. Strains Δric8 (R81a), mcb inl, and Δric8 mcb inl were cultured on VM + inositol plates for 3 days at 25°.
