Figure 7 .

GTP binding of Gα proteins and GEF activity of RIC8. GNA-1 (A), GNA-2 (B), and GNA-3 (C) were incubated with [³⁵S]GTPγS in the absence (●) or presence (▪) of RIC8. All protein concentrations were 200 nM. Reactions were performed in triplicate, as indicated in Materials and Methods. A representative result from several independent assays is shown for each protein.