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. 2011 Sep;189(1):137–151. doi: 10.1534/genetics.111.131227

Figure 1 .

Figure 1 

unc-73 RhoGEF-2 mutants have altered neurotransmitter signaling. (A–C) Animals were examined for paralysis on NGM plates containing 1 mM aldicarb, an acetylcholinesterase inhibitor. unc-73 RhoGEF-2 mutants are weakly resistant to aldicarb compared to wild-type N2 animals and Ex[D1; E]; unc-73(ev802) and Ex[E]; unc-73(ce362) rescued strains. Control egl-8 mutants are moderately resistant to aldicarb. gsa-1(ce81) gain-of-function mutants and gsa-1(ce81) unc-73(ce362) double mutants are hypersensitive to aldicarb. (D and E) Animals were examined for paralysis on NGM plates containing 0.5 mM levamisole, an acetylcholine receptor agonist. unc-73 RhoGEF-2 mutants are hypersensitive to levamisole. Control unc-29 levamisole-sensitive acetylcholine receptor subunit mutants are completely resistant. Error bars indicate SEM. Square brackets denote transgenes present in a particular strain. For example, [D1; E] indicates the unc-73D1 and unc-73E isoform encoding transgenes. (F) Gross synapse morphology is unchanged in unc-73 RhoGEF-2 mutants. SNB-1::CFP (synaptobrevin) localization in dorsal cord cholinergic motor neuron axons [acr-2 promoter (Nurrish et al. 1999)] is similar in unc-73(ce362) mutants and control N2 animals (top). SNB-1::GFP localization in dorsal cord GABAergic motor neuron axons [unc-25 promoter (Hallam and Jin 1998)] is similar in Is[D1]; unc-73(ev802) mutants and control unc-73(ev802)/unc-11 dpy-5 animals (bottom).