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. 2011 Sep;189(1):137–151. doi: 10.1534/genetics.111.131227

Figure 4 .

Figure 4 

Neuropeptide level is reduced in unc-73 RhoGEF-2 mutants. NLP-21::YFP expressed in cholinergic motor neurons was used to examine neuropeptide levels and neuropeptide release. (A) Representative images of DA6 and DB6 cholinergic motor neuron cell bodies in the ventral cord, axons in the dorsal cord, and posterior coelomocytes in wild-type and unc-73(ce362) animals containing unc-129p::nlp-21::yfp. Insets reveal the punctate localization of NLP-21::YFP in axons. (B) Quantification of the arbitrary fluorescence intensity standardized to wild type. No significant difference between mutant and wild-type DA6 and DB6 cell body fluorescence was observed (P = 0.58). YFP fluorescence levels were decreased in unc-73(ce362) dorsal cord axons (**P < 0.001) and posterior coelomocyte cells (*P = 0.01) in comparison to wild type. (C) Coelomocyte uptake of Texas Red-conjugated bovine serum albumin injected into the pseudocoelom is similar in N2 and unc-73(ce362) animals (P = 0.86). Error bars show SEM. P-values were calculated using Wilcoxon’s two-sample test.