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. 2011 Sep;189(1):137–151. doi: 10.1534/genetics.111.131227

Figure 5 .

Figure 5 

Constitutive Gαs/GSA-1 pathway activity rescues unc-73 RhoGEF-2 mutant lethargic locomotion. (A) acy-1(ce2) and gsa-1(ce81) gain-of-function mutations and the kin-2(ce179) loss-of-function mutation suppress Is[D1]; unc-73(ev802), unc-73(ce362), and rab-2(n501) locomotion defects. gsa-1(gf) cDNA expression in heat-shocked (HS) adult mutant animals also rescues unc-73 RhoGEF-2 mutant lethargy. (B) Expression of an acy-1(gf) cDNA exclusively in muscles (M) or the nervous system (NS) fails to rescue the unc-73 RhoGEF-2 lethargic phenotype, although expression of the cDNAs in combination does rescue the lethargy. (C) The unc-73(ce362); egl-3(ok979) locomotion rate is significantly lower than that of either single mutant alone. gsa-1(ce81) rescues the egl-3(ok979) lethargic movement phenotype. Error bars show SEM. **P < 0.001 in comparison to the corresponding unc-73, rab-2, or egl-3 mutant using Student’s t-test.