Figure 1. Increase in OPN in adventitial fibroblasts by Ang II and ALD.
(A) Ang II-induced expression of OPN protein in a dose-dependent manner. The effect of Ang II on OPN expression was observed at 24 h, the concentration for maximal effect of Ang II was observed at 10−7 mol/L. (B) the effects of Ang II on adventitial fibroblasts expression were time-dependent. The effect of Ang II on OPN expression was observed at 10−7 mol/L. The maximal effect of Ang II on OPN expression was observed at 24 h. (C) Adventitial fibroblasts were treated with Ang II (10−7 mol/L) for varying amounts of time following serum deprivation for 48 h. OPN mRNA levels were quantified by real-time RT-PCR and were normalized to β-actin mRNA levels. (D) The effects of Ang II on OPN expression were blocked by the AT1 receptor blocker losartan, whereas the AT2 receptor blocker PD 123,319 had no effect. The gels represent results of 3 independently performed experiments. (E) ALD -induced expression of OPN protein in a dose-dependent manner. The effect of ALD on OPN expression was observed at 24 h. The concentration for maximal effect of Ang II was observed at 10−8 mol/L. (F) the effects of ALD on adventitial fibroblasts expression were time-dependent. The concentration for effect of Ang II was observed at 10−8 mol/L. The maximal effect of ALD on OPN expression was observed at 24 h. Normalized data (bar graphs) are presented as mean ± SEM. * P<0.05 vs. adventitial fibroblasts in DMEM alone (control), ** P<0.05 vs. adventitial fibroblasts in Ang II alone.