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. 2011 Aug 31;11:196. doi: 10.1186/1471-2180-11-196

Figure 2.

Figure 2

Hyd-1 and Hyd-2 activities in iron transport mutants after growth in rich medium. Aliquots of crude extracts (25 μg of protein) derived from each of the mutants grown by fermentation in TGYEP medium, pH 6.5, were separated by non-denaturing PAGE (7.5% w/v polyacrylamide) and stained for hydrogenase activity as described in the Methods section. The stained bands corresponding to active Hyd-1 and Hyd-2 are indicated. The name of the mutants and the corresponding mutated genes are indicated above each lane.