Table 5.
Influence of iron transport mutations on expression of hyaA, hybO and hycA lacZ fusions
| β-Galactosidase specific activity in Miller Units (± standard deviation) | |||
|---|---|---|---|
| Strain/genotypea | Φ(hyaA'-'lacZ) | Φ(hybO'-'lacZ) | Φ(hycA'-'lacZ) |
| MC4100 (wild type) | 818 ± 232 | 52 ± 46 | 44 ± 9 |
| MC4100 aerobically | 12 ± 3 | 12 ± 3 | 13 ± 2 |
| MC4100 + 15 mM formate | 770 ± 535 | 87 ± 30 | 126 ± 57 |
| DHP-F2 (ΔhypF) | 620 ± 221 | 60 ± 27 | 53 ± 22 |
| ΔfecA-E | 633 ± 252 | 52 ± 17 | 41 ± 11 |
| ΔfeoB | 355 ± 96 | 36 ± 7 | 65 ± 40 |
| ΔentC | 410 ± 110 | 40 ± 15 | 33 ± 20 |
| ΔfecA-E feoB | 491 ± 139 | 43 ± 11 | 28 ± 13 |
| ΔentC fecA-E feoB | 371 ± 94 | 45 ± 11 | 35 ± 24 |
| ΔentC feoB | 574 ± 155 | 45 ± 21 | 49 ± 32 |
| ΔentC fecA-E | 340 ± 211 | 47 ± 12 | 57 ± 19 |
a In the interest of clarity only the genotype of the strains is given. The strains used can be found in Table 6. Growth was performed under fermentative conditions in TGYEP, unless indicated otherwise.
n. d.-not determined