Table 6.
Strains and plasmids used in this study
| Strains/plasmids | Genotype | Reference |
|---|---|---|
| MC4100 | F- araD139 Δ(argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150 - | [37] |
| DHP-F2 | MC4100 ΔhypF 59-629AA | [16] |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIqZΔM15 Tn10 (TetR)] | Stratagene |
| PM06 | Like MC4100 but feoB::Tn5 | This study |
| PX06 | Like XL1-Blue but feoB::Tn5 | This study |
| CP411 | Like MC4100 but ΔentC::cat feoB::Tn5 | This study |
| CP413 | Like MC4100 but ΔfecA-E ΔentC::cat feoB::Tn5 | This study |
| CP415 | Like MC4100 but ΔfecA-E ΔentC::cat | This study |
| CP416a | Like MC4100 but ΔentC::cat | This study |
| CP422 | Like MC4100 but ΔfecA-E introduced from GG7 | This study |
| GG7 | W3110 ΔfecA-E::kan | G. Grass |
| CP971 | MC4100 ΔhycAI::kan | [38] |
| CP612 | Like MC4100 but Φ(hyaA'-'lacZ) | This study |
| CP775 | Like MC4100 but Φ(hybO'-'lacZ) | This study |
| CP951 | Like MC4100 but Φ(hycA'-'lacZ) | This study |
| CP1069 | Like MC4100 but ΔhypF Φ(hyaA'-'lacZ) | This study |
| CP1084 | Like MC4100 but ΔhypF Φ(hybO'-'lacZ) | This study |
| CP1149 | Like MC4100 but ΔhypF Φ(hycA'-'lacZ) | This study |
| CP1073 | Like MC4100 but ΔfecA-E Φ(hyaA'-'lacZ) | This study |
| CP1088 | Like MC4100 but ΔfecA-E Φ(hybO'-'lacZ) | This study |
| CP1150 | Like MC4100 but ΔfecA-E Φ(hycA'-'lacZ) | This study |
| CP1075 | Like MC4100 but ΔfeoBb Φ(hyaA'-'lacZ) | This study |
| CP1090 | Like MC4100 but ΔfeoBb Φ(hybO'-'lacZ) | This study |
| CP1151 | Like MC4100 but ΔfeoBb Φ(hycA'-'lacZ) | This study |
| CP1071 | Like MC4100 but ΔentC Φ(hyaA'-'lacZ) | This study |
| CP1086 | Like MC4100 but ΔentC Φ(hybO'-'lacZ) | This study |
| CP1152 | Like MC4100 but ΔentC Φ(hycA'-'lacZ) | This study |
| CP1079 | Like MC4100 but ΔfecA-E feoBb Φ(hyaA'-'lacZ) | This study |
| CP1094 | Like MC4100 but ΔfecA-E feoBb Φ(hybO'-'lacZ) | This study |
| CP1153 | Like MC4100 but ΔfecA-E feoBb Φ(hycA'-'lacZ) | This study |
| CP1081 | Like MC4100 but ΔentC feoBb Φ(hyaA'-'lacZ) | This study |
| CP1096 | Like MC4100 but ΔentC feoBb Φ(hybO'-'lacZ) | This study |
| CP1154 | Like MC4100 but ΔentC feoBb Φ(hycA'-'lacZ) | This study |
| CP1077 | Like MC4100 but ΔentC fecA-E Φ(hyaA'-'lacZ) | This study |
| CP1092 | Like MC4100 but ΔentC fecA-E Φ(hybO'-'lacZ) | This study |
| CP1155 | Like MC4100 but ΔentC fecA-E Φ(hycA'-'lacZ) | This study |
| CP1083 | Like MC4100 but ΔentC fecA-E feoBb Φ(hyaA'-'lacZ) | This study |
| CP1098 | Like MC4100 but ΔentC fecA-E feoBb Φ(hybO'-'lacZ) | This study |
| CP1163 | Like MC4100 but ΔentC fecA-E feoBb Φ(hycA'-'lacZ) | This study |
| Plasmids | ||
| pFEO | feoABC+ from E. coli in pASK-IBA7 | [39] |
| pECD 1079 | feoB+ from E. coli in pASK-IBA7 | N. Taudte and G. Grass |
| pRS552 | KmR ApR lacZ+ lacY+ lacA+ | [20] |
| phyaA552 | like pRS552 but containing Φ(hyaA'-'lacZ) | This study |
| phybO552 | like pRS552 but containing Φ(hybO'-'lacZ) | This study |
| pTL101 | like pRS552 but containing Φ(hycA'-'lacZ), cloned from PstI within hycA to AvaII within hycA | [28] |