Figure 1. Annexin V-staining of 2DG-ABT treated RS(4;11) and HeLa cells.

a, RS(4;11) cells pre-treated with 20 mM fructose or 0–10 mM 2-deoxy-D-glucose in regular RPMI medium containing 12 mM glucose, for 1 hour before the addition of ABT-737 at indicated concentrations. 9 hours later, cells were analyzed by FACS for Annexin-V positivity. Chi Square Test of Independence for two variables, 2DG and ABT, was P(x~₁ ²>193.35) = 0.000000. Since the combination was clearly better than expected values, the statistic suggests synergistic interaction between 2DG and ABT-737. b, HeLa cells were pre-treated for 1 hour either with or without 10 mM deoxyglucose in DMEM containing 12 mM glucose, before adding ABT-737. Six hours later, cells were analyzed by FACS for Annexin-V staining. c, HeLa cells were pre-treated for 3 hours with 2DG before the addition of ABT-737. Cells were assayed for caspase activity at 3 hours after ABT-737 addition. d, HeLa, PPC-1 and MCF-7 cells were treated with 10 mM 2DG for 3 hours before adding 1 µM ABT-263 for overnight incubation. Trypan blue-negative (viable) cells were counted and graphed (mean ± std dev; n = 3). e, Combination therapy reduces clonogenic survival of PPC-1 cells. PPC-1 cells were treated just once for 24 hrs with 5 mM 2DG, 1 µM ABT-263, both, or were left untreated. 250–1000 cells were plated per 30 mm dish, and 12 days later, surviving cell colonies were stained.