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. 2011 Sep 19;6(9):e24626. doi: 10.1371/journal.pone.0024626

Figure 1. Protective effect of single epitope vaccinated mice is not sufficient against lethal virus challenge.

Figure 1

A24Tg mice were immunized i.n. three times at 7 to 9 days intervals with each peptide in the presence of CpG-ODN, PBS alone or CpG-ODN plus empty-liposome solution, and then infected i.n. with lethal dose of A/HK483 (H5N1) (40 PFU/mouse) 1 week after the final immunization. The survival and the body weight were monitored for 14 days (A). On day 5 post-infection, lung viral titers of PA130–138, PB1430–438 or PB2549–557 peptide immunized mice were determined by calculating TCID50 using MDCK cells as described in Materials and Methods (B). *p<0.05. , **p<0.01.