Figure 4. An accumulation of murine CD3⁺ and CD8⁺ cells around the bronchioles in intranasally immunized mice.

A24Tg mice were immunized three times at 7 to 9 days intervals i.n.(A,C,D,E) or s.c.(B) with PA_130–138, PB1_430–438 and PB2_549–557 peptides in the presence of CpG-ODN (B,C,D), Tyrosinase_206–214 plus CpG-ODN (E) or CpG-ODN plus empty-liposome solution (A). Lungs were harvested at day 7 after the final immunization, embedded in O.C.T. compound, frozen in dry ice-2-propanol. Ten µm thick frozen sections were prepared. The sections were post-fixed in acetone∶ethanol (1∶1) solution and blocked endogenous avidin and biotin activity, then stained with anti-mouse CD3 (A,B,C) or anti-mouse CD8α (D,E).