Figure 3. CLSM micrographs for human squamous carcinoma COLO 16 cell line.

Cell cultures were treated with 50 ng/ml, 100 ng/ml and 200 ng/ml of RRM-MV for 3 h in A, B, and C respectively. Cell culture in D was treated with 200 ng/ml of RRM-C, while cell cultures in E were similarly incubated without any treatment. More necrotic cells and detachment can be seen in B and C as compared with A indicating dose-dependent cytotoxic effect of RRM-MV. No cellular detachment can be seen in the cell culture treated with 200 ng/ml of the negative control RRM-C in D or in the non-treated cell culture in E.