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. 2011 Sep 19;6(9):e24809. doi: 10.1371/journal.pone.0024809

Figure 8. The effect of RRM peptide treatment on total Akt and p- Akt in B16F0 cell line.

Figure 8

A. Western blots for total Akt (pan) Rabbit MAB in I and p-Akt (Thr308) Rabbit MAB in II without Akt inhibitor. B. Western blots for B16F0 cells treated with 50 µM LY294002 (PI3 kinase inhibitor) prior to immunoblotting with total Akt rabbit MAB in III and p-Akt (Thr308) rabbit MAB in IV. In A, Cells were grown in DMEM only in lane 1; DMEM and 800 ng/ml RRM-C in lane 2; and DMEM with 800 ng/ml RRM-MV in lane 3. In B, cells were either grown in DMEM in lane 1, or in DMEM with 50 µM LY294002 for 1 h in lane 2. Similar intensities of the 60 kDa immune bands for total Akt and p-Akt in treated and non treated cells in A indicate the lack of effect of the RRM-designed peptides on p-Akt activity as compared with the inhibitory effect of the Akt inhibitor on p-Akt activity in B.