Table 1. Culture pH of laboratory and vineyard yeast in synthetic defined medium.
Strain | SC 2% Glu | SC 0.5% Glu | SC 0.05% Glu | SC 2% Glu+Cit-Phos Buffer | YEPD |
BY4743 | 2.62 | 3.26 | 6.06 | 4.99 | 4.18 |
BY4742 | 3.01 | 3.68 | 6.24 | 5.16 | 4.42 |
BY4741 | 3.41 | 4.66 | 6.18 | 5.66 | 5.04 |
RM11 diploid | 2.65 | 3.18 | 5.36 | 5.14 | 4.52 |
RM11 MAT a | 2.68 | 3.34 | 5.26 | 5.11 | 4.47 |
RM11 MATĪ± | 2.67 | 3.34 | 5.41 | 5.13 | 4.51 |
Cells were grown in synthetic complete medium (SC) with the indicated carbon source or rich YEPD medium, and pH was determined after 48 hours. For growth in buffered medium (Cit-Phos buffer), a citrate phosphate buffer (64.2 mM Na2HPO4 and 17.9 mM citric acid) adjusted to pH 6.0 was added to the medium prior to inoculation. Initial pH values for synthetic complete and YEPD were 4.0 and 6.6, respectively.