Figure 3. CpG islands 5′UPS of mir-34b are hypermethylated in clinical melanoma samples.

CpG island methylation in the putative mir-34b promoter was measured by bisulfite genomic sequencing (conventions are as appeared in Figure 2). Six clones from each sample were tested. Patient samples are annotated as follows: (P) = primary melanoma, (R) = regional metastasis, (D) = distant metastasis, (N) = nodal metastasis.