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. 2011 Sep 19;6(9):e24922. doi: 10.1371/journal.pone.0024922

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Mazar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Wound healing assay for melanoma cells. A) and B) Representative images of WM1552C/VO and WM1552C/34b cells or A375/VO and A375/34b cells. Images were taken between 0 and 20 hrs after scratch formation. C) and D) Quantitation of experiments shown in (A) and (B), performed in triplicate. The percent surface area between the wounds was calculated using NIS elements software. Each time point was compared with the 0 hr time point of the respective cell line. Asterisks indicate statistical significance by Kruskal Wallis nonparametric test, P<0.005.