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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Sex Med. 2010 Nov 29;8(2):437–446. doi: 10.1111/j.1743-6109.2010.02128.x

Figure 4.

Figure 4

CXCL5 activates JAK/STAT time-dependently and with specificity.

(A) Schwann cell line RT4-D6P2T was treated with CXCL5 at 50 ng/ml for the indicate time and then analyzed by western blot for the expression of STAT1, phosphorylated STAT1 (pSTAT1), STAT3, and phosphorylated STAT3 (pSTAT3). β-actin served as control. (B) Same as in (A) except that AG490 was added to a final concentration of 100 nM prior to the addition of CXCL5. (C) The ratio (in percentile) of pSTAT1 versus STAT1 expression for each treatment was determined by dividing the densitometric values of these two protein bands obtained from the western blots. Each bar represents the average of 3 independent experiments. (E) Same as in (C) except that the proteins are pSTAT3 versus STAT3.