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. 2011 Sep 19;6(9):e25072. doi: 10.1371/journal.pone.0025072

Figure 3. Comparison of larvae and adult diurnal gene expression. QPCR analysis of candidate circadian gene expression in larvae.

Figure 3

Larvae and adult tissues were compared using QPCR, to determine similar patterns of gene expression over a 12∶12 LD treatment (a. clock, b. cryptochrome 1, c. cryptochrome 2, d. cycle, e. eyes absent, f. timeless). Relative fold changes in RNA expression levels (mean ± SEM of triplicate QPCR reactions) are presented for both 2009 larvae (primary y-axis) and adult tissue (secondary y-axis). Shaded areas represent the 12 hours of darkness for larvae (12∶12 LD) and 11 hours of darkness for adult tissue (13∶11 LD).