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. 2011 Sep 19;6(9):e25174. doi: 10.1371/journal.pone.0025174

Figure 4. In vivo binding of AEBP2 and EZH2 and the methylation level of H3K27me3 on the NCC-associated genes.

Figure 4

The cross-linked chromatin from 14.5-dpc embryos was precipitated with the anti-AEBP2, EZH, and H3K27me3 antibodies (left, middle, right). The PCR products from ChIP were presented in the following order: Input, IgG (pre-immune serum), AEBP2, EZH2-AB, or H3K27me3. The majority of the genes involved in the RET and EDNRB pathways, except Zfhx1b, are bound by AEBP2 and EZH2, and also modified with H3K27me3.