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. 2011 Sep 19;6(9):e25174. doi: 10.1371/journal.pone.0025174

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The levels of AEBP2 and EZH2-binding to the NCC-associated genes were compared between the wild-type (blue) and Aebp2^+/β-Geo (red) embryos with qPCR using the immunoprecipitated DNA derived from 14.5-dpc embryos (A,B). The methylation levels of H3K27me3 was also compared between the two types of embryos (C). Potential involvement of the PRC1 was also tested using RING1B antibody (D). The amount of each precipitated DNA is presented as a relative value (%) to that of the input DNA (y-axis). The values derived from the wild-type and Aebp2^+/β-Geo embryos are presented together per each gene (x-axis).