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. 2011 Sep 19;6(9):e25143. doi: 10.1371/journal.pone.0025143

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Twelve mice were randomly assigned to PBS group and Act B group (n = 6). Immunohistochemistry indicated increased numbers of p-JNK-positive (A) and p-cJun-positive cells (C) at the wounded skin on day 3 and day 5 after activin B treatment, as compared with PBS control. Enlarged images of the boxed area were presented on the right side. Scale bars: 100 µm. Quantified percentages of p-JNK and p-cJun immunopositive cells are presented in (B) and (D) respectively. (E, F) Western blotting showed the level of JNK phosphorylation reached the highest value on day 3 and higher than PBS group on day 1 to day 5. 3 h, 6 h, 12 h: 3, 6 and 12 hours after wound. *P<0.05, **P<0.01, compared with PBS control.