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The optimal numbers of individuals on average detection probabilities of variants of P = 0.005, 0.01, and 0.025 with coverage depths C = 20, 30, and 40 and threshold T = 3. The hyperparameters 2.89 and 4.76 are estimated by the PMLE and PMME, respectively. The number on each curve is the optimal number of individuals. PMLE, pseudo maximum-likelihood estimator; PMME, pseudo method of moments estimator.
