FIG. 4.

Effect of TN on cell shortening in isolated cardiomyocytes from WT and MyAkt mice. Murine cardiomyocytes were incubated with TN (3 μg/ml) for 5–6 h in vitro before assessment of mechanical properties. A cohort of WT cardiomyocytes were coincubated with the ER stress inhibitor TUDCA (500 μM) alone with TN. (A) Representative contractile traces in cardiomyocytes from WT mice in the absence or presence of TN or TUDCA; (B) representative contractile traces in cardiomyocytes from MyAkt mice in the absence or presence of TN; (C) resting cell length; (D) PS (% of resting cell length); (E) maximal velocity of shortening (+dL/dt); (F) maximal velocity of relengthening (−dL/dt); (G) TPS; and (H) time-to-90% relengthening (TR₉₀). Mean±SEM, n=80 cells per group, *p<0.05 versus WT group; ^#p<0.05 versus WT-TN group.