FIG. 7.

Effect of TN on reactive oxygen species (ROS) and cell survival in cardiomyocytes from WT and MyAkt mice. Murine cardiomyocytes were incubated with TN (3 μg/ml) for 5–6 h in vitro before examination. A cohort of WT cardiomyocytes were coincubated with the ER stress inhibitor TUDCA (500 μM), the antioxidant catalase-polyethylene glycol (PEG, 15,000 IU/ml), or the mitochondrial permeation pore (mPTP) inhibitor cyclosporin A (200 nM) at the same time of TN exposure. (A). Representative DCF fluorescent images depicting cardiomyocytes from WT and MyAkt mice treated with TN or TN-treated WT cells in the presence of the ER stress inhibitor TUDCA (500 μM). A cohort of WT cardiomyocytes were coincubated with H₂O₂ (100 μM, positive control) for 5 h in the absence or presence of the antioxidant catalase-PEG; (B) ROS production; and (C) MTT cell survival. Mean±SEM, n=5–8 isolations per group, *p<0.05 versus WT group; ^#p<0.05 versus WT-TN group. (To see this illustration in color the reader is referred to the web version of this article at www.liebertonline.com/ars).