Table 1.
Oligonucleotide primer pairs and the PCR conditions used
| Gene | Primer Sequence (5’ to 3’)* | Annealing Temperature |
Size (bp) |
Antibiotic resistance |
Reference |
|---|---|---|---|---|---|
| tetM | GTGGACAAAGGTACAACGAG CGGTAAAGTTCGTCACACAC |
55°C | 406 | Tetracycline | (36) |
| tetW | GAGAGCCTGCTATATGCCAGC GGGCGTATCCACAATGTTAAC |
64°C | 168 | Tetracycline | (37) |
| tetQ | TTATACTTCCTCCGGCATCG ATCGGTTCGAGAATGTCCAC |
55°C | 904 | Tetracycline | (36) |
| blaTEM-1 | CCAATGCTTAATCAGTGAGG ATGAGTATTCAACATTTCCG |
55°C | 858 | Penicillin | (38)** |
| blaZ | CAGTTCACATGCCAAAGAG TACACTCTTGGCGGTTTC |
54°C | 846 | Penicillin | (2) |
| cfxA | GCAAGTGCAGTTTAAGATT GCTTTAGTTTGCATTTTCATC |
58°C | 802 | Penicillin | (39) |
| van(A) | ATGAATAGAATAAAAGTTGCAATAC CCCCTTTAACGCTAATACGAT |
62°C | 1029 | Vancomycin | (40) |
| van(D) | TGTGGGATGCGATATTTCAA TCGACGCAAGTATCCGGTAA |
54°C | 500 | Vancomycin | (30) |
| van(E) | TGTGGTATCGGAGCTGCAG GTCGATTCTCGCTAATCC |
52°C | 513 | Vancomycin | (41) |
| 16S rRNA gene | AGA GTT TGA TCC TGG CTC AG ACG GCT ACC TTG TTA CGA CTT |
56 °C | 1500 | All bacteria | (5) |
*
The top primer is the sense primer, and the bottom is the antisense primer.
**
With the exception of the annealing temperature which was modified.