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. 2011 Sep 20;2011:674149. doi: 10.1155/2011/674149

Figure 2.

Figure 2

Time-dependent formation of Cu(II)-GSSG after the addition of Tempol to a Cu(I)-[GSH]2 complex. Tempol was added to a solution containing the Cu(I)-[GSH]2 complex and the mixture incubated at 37°C for 0–90 min. Samples were taken during the incubation and the increase in OD625 nm registered. Results are expressed as mM Cu(II)-GSSG concentration. The symbols represent: (♦) Cu(II)-GSSG (5 mM); (■) GSH (15 mM); (○) Cu(I)-[GSH]2 (5 mM); (●) Cu(I)-[GSH]2 (5 mM) plus Tempol (4 mM).