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. 2011 Sep 20;2011:674149. doi: 10.1155/2011/674149

Figure 3.

Figure 3

Effect of EDTA on the recovery of GSSG from the Cu(II)-GSSG complex assayed by the NADPH/glutathione reductase assay. NADPH (0.2 mM), glutathione reductase (2 U/mL), and EDTA (2 mM) were added to a solution containing GSSG (36 μM) or a preformed Cu(II)-GSSG complex (36 μM). The decrease in OD340 nm was registered and the results expressed as μM GSSG concentration. The asterisk on the bars indicate that such values are statistically different, at the level of a P < 0.001, from all other values.