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Assessment of GSSG by the EDTA-modified NADPH/glutathione reductase assay: correlation between GSSG and Cu(II)-GSSG. NADPH (0.2 mM), glutathione reductase (2 U/mL), and EDTA (2 mM) were added to solutions containing increasing concentrations of the Cu(II)-GSSG complex (5–60 μM). Results are expressed as μM GSSG concentration. The symbols represent: solutions containing the Cu(II)-GSSG complex (5–60 μM) assayed in the presence (■) or in the absence of EDTA (□).
