Figure 7.
(a) Effect of the exposure of Cu(I)-[GSH]2 to an exogenous source of superoxide radicals on the thiol-titratable groups and EDTA-releasable GSSG. Hypoxanthine (0.2 mM) plus xanthine oxidase (XO; 2 U/mL) were added to a solution containing the Cu(I)-[GSH]2 complex (10 μM), and the mixture was incubated for 0–60 min, at 30°C. Samples were taken from the incubation media after 1, 30, and 60 min and analyzed for their TTG (light grey bar) and EDTA-releasable GSSG (dark grey bar) levels. Results are expressed as GSH equivalents (μM). (b) Effect of the exposure of Cu(I)-[GSH]2 to an exogenous source of superoxide radicals on the concentration of copper (I). Hypoxanthine (0.2 mM) plus xanthine oxidase (XO; 2 U/mL) were added to a solution containing the Cu(I)-[GSH]2 complex (10 μM) and the mixture was incubated for 0–60 min, at 30°C. Samples were taken from the incubation media after 1, 30, and 60 min and analyzed for their Cu(I) concentration. Results are expressed as Cu(I)-bathocuproine (μM). The asterisk on the bars indicate that such values are statistically different, at the level of a P < 0.001, from all other values.