Table 1. Fluorescence and cellular uptake of formulated Dbait.
Molecules/method | Dbait | Chloroquine | Fluorescencea | MCC | Cor MCC | MCC | Cor MCC |
---|---|---|---|---|---|---|---|
(μg ml−1) | (100 mg ml−1) | × 106 μg−1ml−1 | 5 h | 5 h | 24 h | 24 h | |
— | — | − | 0 | 3 | ND | 3 | ND |
— | — | + | 0 | 3 | ND | ND | ND |
Electroporation | 1.6 | − | 6.17 | 9 | 1 | 11 | 2 |
Dbait | 1.6 | − | 6 | 3 | 0.4 | 3 | 0.5 |
Dbait/PEI11K | 1.6 | − | 1.92 | 26 | 14 | 13 | 7 |
Dbait/bPEI25K | 1.6 | − | 3.04 | 52 | 17 | 58 | 19 |
Dbait/PEI22K | 1.6 | − | 3.32 | 54 | 16 | 43 | 13 |
Dbait/Superfect | 1.6 | − | 6.41 | 220 | 34 | 204 | 32 |
Dbait/Superfect | 1.6 | + | 6.41 | 218 | 34 | ND | ND |
coDbait | 1.6 | − | 3.47 | 10 | 3 | ND | ND |
coDbait | 1.6 | + | 3.47 | 21 | 6 | ND | ND |
coDbait | 16 | − | 3.47 | 66 | 19 | 64 | 18 |
coDbait | 16 | + | 3.47 | 236 | 68 | 214 | 62 |
coDbait | 32 | − | 3.47 | 145 | 42 | ND | ND |
coDbait | 32 | + | 3.47 | 390 | 112 | ND | ND |
Abbreviations: Cor MCC, corrected cellular content FL2/fluorescence; MCC, mean cellular content FL2 value (>3 experiments); ND, not defined.
Fluorescence at FL2 value.