Table 1. List of predicted ORFs in contigs expressed in the NS.
HVS group | Contigs | Predicted ORFs |
---|---|---|
HVS-1 | 022, 030, 058, 110, 141, 151, 198, 225, 246 | HP |
078, 209 | CHP | |
126, 152 | CHP, HP | |
008, 113 | HP, Zn-finger | |
012 | Zn-finger, HP, CHP | |
029 | RHH protein, CHP | |
038 | HP, terminase | |
042 | CHP, Zn-finger | |
061 | Transcription factor, HP | |
070 | RHH protein, integrase | |
096 | HP, envelope phage protein | |
140 | HP, transposase | |
178 | Replicative ATPase | |
193 | Integrase | |
208 | HP, methyltransferase | |
HVS-2 | 014, 024, 027, 041, 065, 106, 120, 128, 138, 162, 179, 194, 200, 202, 218, 228, 242, 247 | HP |
055, 088, 107 | Membrane, HP | |
066, 090, 237 | Membrane | |
067, 199 | CHP | |
127, 129 | HP, CHP | |
163, 190 | HP, methyltransferase | |
011 | Terminase, HP | |
049 | Membrane, CHP, tat-signal protein | |
059 | Zn-finger, CHP | |
062 | Prohead peptidase, capsid structure | |
069 | HP, phosphohydrolase | |
072 | Membrane, CHP | |
104 | Terminase | |
123 | SsDNA-binding protein, HTH-protein, HP | |
213 | Phage mu-like | |
214 | HP, transcriptional regulator | |
261 | Deoxyribosyltransferase | |
HVS-3 | 017, 031, 144, 176, 221, 226, 248 | HP |
171, 116 | Membrane, HP | |
236, 251 | Membrane | |
044 | MCM protein (DNA replication initiator) | |
060 | Replicative DNA helicase (intein endonuclease) | |
076 | HNH endonuclease, HP | |
080 | HP, ssDNA-binding protein | |
084 | HTH phage repressor (peptidase) | |
114 | LAGLIDADG endonuclease | |
130 | CHP | |
137 | Methyltransferase, phosphoadenosine reductase | |
150 | Ssl1-like protein (transcription factor) | |
227 | Hypothetical DUF protein | |
249 | HNH endonuclease, membrane | |
HVS-4 | 006, 036, 047, 050, 063, 098, 118, 149 | HP |
005, 035, 074 | HP, CHP | |
001 | HP, capsid, methyltransferase | |
004 | HP, CHP, terminase, methyltransferase | |
007 | Methyltransferase, nuclease, HP | |
009 | HP, traslocation domain protein | |
019 | Terminase, HP | |
043 | HP, transposase | |
068 | Lamgl protein (glucanase) | |
113 | HP, homeodomain protein | |
125 | Membrane, HP | |
158 | WD40/YVTN repeat-like domain | |
165 | HP, mRNA autoregulator | |
174 | Arginine N-succinyltransferase | |
HVS-5 | 083, 131, 132, 142, 164, 169, 173, 216, 255 | HP |
002, 015 | HP, CHP, membrane | |
026, 182 | Phage DNA primase | |
160, 266 | Membrane | |
075 | HP, membrane | |
081 | Hypothetical ParB nuclease-like protein | |
111 | Terminase | |
124 | Replicative DNA-dependent ATPase | |
167 | HP, CHP | |
212 | CHP |
Abbreviations: CHP, conserved hypothetical protein; DUF, domain of unknown function; HP, hypothetical protein; HTH, helix-turn-helix domain; HVS, halophilic viral sequence; NS, natural sample; ORF, open reading frame; RHH, ribbon-helix-helix domain; ssDNA, single-stranded DNA.