Figure 3.—

 (A) N. benthamiana protoplasts infected by TuMV-GFP. Fluorescence micrograph at 48 hr post-transfection showing GFP expression in the fraction of protoplasts that resulted infected with TuMV-GFP. (B) Accumulation dynamics of (+) TuMV RNA in N. benthamiana protoplasts transfected with a plasmid expressing the replication-defective TuMV-VNN mutant. Plot of TuMV (+) RNA molecules in 100 ng of plant total RNA vs. time in four independent transfection experiments. Each experimental data correspond to the average of three RT–qPCR determinations. Different experiments are represented with different colors. Solid lines represent the best fit of Equation 7 to the control data.