Table 3.

pch2Δ and pch2Δ rad54Δ display similar meiotic crossover levels

	Total spores	Recombinant	Parental	% recombinant
	Chromosome III
HIS3-LEU2
Wild type	2711	351	2360	12.9
pch2∆	2691	389	2302	14.5
pch2∆ rad54∆	458	62	396	13.5
LEU2-CEN3
Wild type	2711	184	2527	6.8
pch2∆	2691	241	2450	9.0
pch2∆ rad54∆	458	41	417	9.0
CEN3-MAT
Wild type	2711	402	2309	14.8
pch2∆	2691	374	2317	13.9
pch2∆ rad54∆	458	65	393	14.2
Chromosome VII
TRP5-CYH2
Wild type	2711	908	1803	33.5
pch2∆	2691	1149	1542	42.7
pch2∆ rad54∆	458	199	259	43.4
CYH2-MET13
Wild type	2711	260	2451	9.6
pch2∆	2691	469	2222	17.4
pch2∆ rad54∆	458	81	377	17.7
MET13-LYS5
Wild type	2711	559	2152	20.6
pch2∆	2691	747	1944	27.8
pch2∆ rad54∆	458	139	319	30.3
Chromosome VIII
CEN8-THR1
Wild type	2711	606	2105	22.4
pch2∆	2691	649	2042	24.1
pch2∆ rad54∆	458	116	342	25.3
THR1-CUP1
Wild type	2711	668	2043	24.6
pch2∆	2691	948	1743	35.2
pch2∆ rad54∆	458	123	335	26.9

Wild-type (NH942/NH943), pch2Δ (EAY2209/EAY2210), and pch2Δ rad54Δ (EAY2681/EAY2685) strains were sporulated and analyzed for segregation of genetic markers in the NH942/NH943 strain background. Crossover frequencies in this strain were calculated from recombination frequencies in spores as described previously (Zanders and Alani 2009). Data for wild type and pch2Δ are from Zanders and Alani (2009). Spore viability was 91% for wild type (n =743 tetrads dissected), 97% for pch2Δ (n = 707 tetrads), and 45% for pch2Δ rad54Δ (n = 256 tetrads).