Table 4.
Spore formation efficiency and viability in mek1-as and MEK1-GST mutants
| Genotype | % sporulation | No. analyzed | % spore viability | Spores analyzed |
| Wild type | 79.1 | 436 | 93.5 | 400 |
| pch2∆ | 80.9 | 429 | 95.3 | 400 |
| spo11-HA | 81.1 | 434 | 92.5 | 400 |
| mek1-as | 84.7 | 163 | 98.8 | 80 |
| MEK1-GST | 66.9 | 178 | 87.5 | 160 |
| pch2∆ spo11-HA | 74.9 | 453 | 56.8 | 400 |
| mek1-as spo11-HA | 87.8 | 164 | 92.3 | 400 |
| mek1-as pch2Δ | 88.5 | 191 | 92.0 | 400 |
| MEK1-GST spo11-HA | 69.8 | 162 | 85.4 | 546 |
| MEK1-GST pch2Δ | 67.5 | 155 | 86.5 | 408 |
| pch2Δ spo11-HA mek1-as | 85.8 | 183 | 40.8 | 400 |
| pch2Δ spo11-HA MEK1-GST | 63.5 | 143 | 89.0 | 552 |
Sporulation efficiencies for the above strains were counted after 5 days on sporulation media at 30°. Tetrads were dissected on YPD and scored for spore viability after 3 days.