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. Author manuscript; available in PMC: 2011 Sep 20.
Published in final edited form as: Int J Oncol. 2011 Mar 17;38(6):1511–1520. doi: 10.3892/ijo.2011.980

Figure 4.

Figure 4

Activation of FAM84A promoter by CAR. The -9.0-kb FAM84A-Luc reporter plasmid was co-transfected with CAR expression plasmid into HepG2 cells for 24 h. Subsequently, these cells were treated with DMSO, CAR activator (250 nM TCPOBOP) or CAR repressor (8 μM androstenol) for an additional 48 h. Then whole cell extracts were prepared and used to measure luciferase activities, which were normalized against the values of Renilla luciferase activities. The activities of the FAM84A promoter are reported as the mean ± SD, n=3 with the activity of DMSO-treated cells as one. The -1.8-kp CYP2B6 reporter plasmid was used in a similar experiment as a positive control for CAR-mediated activation.