Intra-hepatic localization of FAM84A protein in PB-treated mice. (A) Liver sections were prepared the DEN/6 weeks PB-treated or DEN-treated Car+/+ C3He mice and subjected to immunohistochemical analysis using an anti-FAM84A serum and pre-bleed serum as control. CV and PV denote central and portal veins, respectively. FAM84A protein was induced in the PB-treated mouse liver around the CV regions, not the PV regions. (B) Using laser capture microdissection, cells around CV and PV were collected in the liver sections of C3He male mice treated with PBS or PB for 24 h. These RNAs were subsequently used for real-time PCR to measure FAM84A mRNA levels in the PBS- or PB-treated samples as shown by open and closed bars, respectively. The relative levels of FAM84A mRNA were calculated by setting the levels in the CV of PBS-treated C3He mice as one. The data are reported as the mean ± SD, n=3.