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Inpact of fluorescence quenching: (a) bright-field, (b) SONICC and (c) TPE-UVF measurements of catalase crystals, with bright SHG images being generated at low power but minimal TPE-UVF signal being generated even at incident powers of >40 mW and an acquisition time of 2 min. This trend is attributed to quenching of the TPE-UVF signal by the heme chromophore in catalase.
