Figure 5.

Comparison of one- and two-photon excited UVF of an ABC maltose transporter membrane-protein crystal: (a) bright-field and (b) TPE-UVF measurements completed in situ through the crystallization-plate cover slip; (c) shows a corresponding line scan analysis showing high signal to background for moderate laser power. (d) Bright-field and (e) conventional UVF measurements on the same crystals performed after breaking the well seal and extracting a crystal to allow UVF. (f) Corresponding line scan analysis indicates weaker signal to background compared with TPE-UVF measurements.