Table 3. Data-processing statistics for eight tetragonal lysozyme crystals: four native (i.e. nitrate-free) crystals and four soaked in 100 mM nitrate.
Values in parentheses are for the highest resolution shell (2.0–1.9 Å). Indicated doses are typical values used at 100 and 295 K.
| Crystal | T (K) | Dose (MGy) | Unique reflections | Multiplicity | Completeness (%) | I/σ(I) | Rmerge (%) | Wilson B value (Å2) | Imean (SCALA) | I/I0 (SCALA) | Unit-cell parameters (Å) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Native | 100 | Fresh | 10042 | 6.8 (6.8) | 100.0 (100.0) | 5.0 (7.0) | 6.0 (8.3) | 14.4 | 6853 | 1.00 | a = 79.77, c = 37.54 |
| Native | 100 | 1.9 | 10063 | 6.8 (6.8) | 100.0 (100.0) | 4.9 (6.7) | 6.2 (8.7) | 15.8 | 5729 | 0.84 | a = 79.85, c = 37.56 |
| Native | 295 | Fresh | 10369 | 6.9 (7.0) | 100.0 (100.0) | 4.3 (4.3) | 5.9 (12.3) | 18.7 | 1458 | 1.00 | a = 80.15, c = 38.44 |
| Native | 295 | 0.12 | 10385 | 6.9 (7.0) | 100.0 (100.0) | 4.2 (3.6) | 6.3 (16.8) | 21.2 | 1117 | 0.77 | a = 80.19, c = 38.44 |
| Nitrate | 100 | Fresh | 10006 | 6.8 (6.9) | 100.0 (100.0) | 6.5 (3.7) | 7.3 (14.3) | 16.4 | 3685 | 1.00 | a = 79.56, c = 37.61 |
| Nitrate | 100 | 1.9 | 10046 | 6.8 (6.9) | 100.0 (100.0) | 6.7 (4.4) | 6.8 (13.6) | 17.1 | 2998 | 0.81 | a = 79.68, c = 37.66 |
| Nitrate | 295 | Fresh | 10397 | 6.9 (7.0) | 100.0 (100.0) | 4.1 (3.6) | 6.8 (16.3) | 19.7 | 1660 | 1.00 | a = 80.05, c = 38.64 |
| Nitrate | 295 | 0.12 | 10377 | 6.9 (7.0) | 100.0 (100.0) | 3.8 (3.2) | 7.3 (20.6) | 20.7 | 1466 | 0.88 | a = 79.88, c = 38.74 |