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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: Cell. 2011 Sep 2;146(5):826–840. doi: 10.1016/j.cell.2011.07.020

Figure 1. Development of qPTPome and q-oxPTPome.

Figure 1

(A) Scheme for each assay. (B) NIH3T3 cells were lysed in presence of DTT, applied to a gel filtration column, treated with PV or left untreated, and then analyzed by immunoblotting with the oxPTP Ab. (C) Native and denatured PV-treated lysates from (B) were immunoprecipitated with oxPTP or Ptpn11 antibodies, as indicated, and analyzed by immunoblotting. (D) PTPs in NIH3T3 cells, as identified by qPTPome. (E) Venn diagram demonstrating reproducibility of the qPTPome assay (n=3). See Figure S1 for additional variations of these assays.

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