Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Biomol Screen. 2011 Jan 18;16(2):230–238. doi: 10.1177/1087057110392038

FIG. 5.

FIG. 5

The EC50 of hits from high-throughput screening. Dose-response experiments for the hits from the screen and the small-molecule GyrB inhibitor Vertex-13 were performed using the fluorescence polarization (FP) assay to monitor the inhibition of the binding of 40 nM Novo-TRX to 40 nM GyrB. The average FP signal for each quadruplicate was calculated and normalized to the DMSO control sample. The error bars represent standard deviation of 4 replicates. The EC50 values were determined using the sigmoidal dose-response equation in GraphPad Prism 4.