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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Neuroendocrinol. 2011 Jun;23(6):531–541. doi: 10.1111/j.1365-2826.2011.02131.x

Figure 1.

Figure 1

(A) Acute alcohol (EtOH) increases c-fos levels in CRF-positive cells of the PVN. Bright-field photographs through the PVN from vehicle controls (top), and rats challenged with alcohol (bottom). Images show a representation of the double immunohistochemical techniques that stained c-fos-ir in black nuclei and CRF-ir in brown cytoplasm with a 10X dry objective (scale bar = 200 µm) and insets with a 40X objective (scale bar = 50 µm). (B) Cell counts were obtained for c-fos-ir and CRF-ir positive cells throughout the rostral-caudal extent of the PVN. Mean ± SEM levels of c-fos signals in CRF–immunoreactive cells in the PVN (top right) and total number of CRF cells (bottom right) of 5–6 rats injected with vehicle or EtOH. ***, P<0.001.