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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Neuroendocrinol. 2011 Jun;23(6):531–541. doi: 10.1111/j.1365-2826.2011.02131.x

Figure 2.

Figure 2

(A) Acute alcohol (EtOH) increases c-fos signals in the A1–A2/C1–C3 cell region, identified by DBH. Bright-field photographs through the A1–A2/C1–C3 area of brain stem from vehicle controls (left), and rats challenged with alcohol (right). Images show a representation of the double immunohistochemistry that stained c-fos-ir in black and DBH-ir in brown (scale bar = 200 µm). (B) Cell counts were obtained for c-fos immunoreactivity in DBH-ir cells. Mean ± SEM levels of c-fos signals in DBH–ir cells in the A1/C1, A2/C2 and C3 area of 5–6 rats injected with vehicle or alcohol. *, P<0.05; ***, P<0.001.