Figure 3.
(A) Acute alcohol (EtOH) increases c-fos signals in the LC, identified by TH-positive cells. Bright-field photographs through the LC from vehicle controls (top), and rats challenged with alcohol (bottom) with a 20X objective (scale bar = 100 µm) (left) and a 60X objective (scale bar = 50 µm) (right). (B) Cell counts of c-fos positive nuclei in TH-positive cells in the LC. Mean ± SEM levels of c-fos positive signals in TH–immunoreactive cells in the LC of 5–6 rats injected with vehicle or alcohol (right). **, P<0.01.